GR and BRG1 cistromes of murine primary bone marrow derived macrophages treated with LPS and Dex+LPS [ChIP-seq]

We mapped the genome wide binding profile of the chromatin remodeller BRG1 in wild type bone marrow derived macrophages (BMDMs) after 3h LPS and LPS plus Dex treatment by ChIPseq. Additionally, we profiled the GR cistrome in Dex + LPS-treated BMDMs after 3h treatment by ChIPseq. Overall design: We performed ChIP sequencing against GR in BMDMs treated for 3 hours with 100 ng/ml LPS and 1 mM Dex. Furthermore, we profiled the genomic occupancy of BRG1 in BMDMs treated with 100 ng/ml LPS or 100 ng/ml LPS plus 1 mM Dex for 3 hours. Two biological replicates were submitted for sequencing for each epitope and treatment conditions.