Characterization of liver immune cell subsets of primary biliary cholangitis (PBC) mouse models using single-cell RNA sequencing. Characterization of liver immune cell subsets of primary biliary cholangitis (PBC) mouse models using single-cell RNA sequencing

We characterized difference immune cell subsets in liver microenvironment between PBC mouse models (mainly dnTGFβRII mice) and wild type mouse by transcriptome and proteome single cell squencing, and discovered an unconventional cytotoxic CD8αα T cells increased in dnTGFβRII mice. Overall design: CD45+ immune cells sorted from liver of wild type mice and PBC mouse models, including dnTGFβRII mice, IFN-γ ARE-Del-/- mice and P40-/-IL-2Ra-/- mice.