A. Protein topology showing the presence of signal peptide and/or transmembrane helices in the protein signal peptide spans from 1–34 amino acids, whereas transmembrane domain spans from 7–29 amino ac
*Total protein amount calculated as sum of GMMA-associated protein and soluble protein.#Total protein amount measured directly by Bradford assay.S. sonnei ΔtolR ΔgalU was grown in HTMC in a 5 L fermen
(A) SDS-PAGE and Coomassie blue staining of the whole cell lysate corresponding to E. coli BL21 transformed with pCold-TF-AcLAP plasmid (lane 1), soluble fraction of the sonicated cell lysate (lane 2)
