Dataset: Live imaging and quantification of circulating potentially metastatic tumor cells in early pupal stage of Drosophila melanogaster.

Movie 1. 2-Dimensional confocal projections showing circulatory tumors cells (CTCs) during early Drosophila pupal leg and wing development. Schematic (A) and 2D projections (B-D) show the movement of CTCs during morphogenesis. Panel A shows schematically the region imaged (dotted line). Panel B shows only the green channel, where invasive tumor cells (RasV12; scrib1) are marked with GFP. Panel C shows a composite of the green and DIC channels. This panel, in addition to showing tumor cells, also allows for observing the developing legs and wings. Panel D shows the composite of the green and DIC as well as various labels that allow for the direction of CTCs to be observed. CTCs are labelled within circles of various colors. Arrows indicate the direction of CTC movement. Sections of developing leg are labelled with blue lines. The distal boundaries of leg and wing imaginal discs are labelled with yellow lines.