Temporal single cell and bulk RNA sequencing data of adult mouse spinal cord after injury

For single cell RNA sequencing dataset, the 10×
Genomics platform was used to perform massive single cell mRNA profiling from
mouse spinal cord samples collected at 6 different time points after Spinal
Cord Injury (crush injury), i.e., 4h, 1d, 3d, 7d, 14d, and 38d post injury. Uninjured
spinal cord samples are included as well. Both male and female C57BL/6 mice
were used in the analyses, 10mm-long spinal cord segments encompassing the
lesion site were dissociated into single cells through our proprietary method
developed based on published studies1,2. For non-injury controls,
male and female spinal cord samples were sequenced separately, and male and
female scRNA-seq data overlapped rather well.

For bulk RNA sequencing, 1 cm long spinal cord segments
centered on leison core, average of 20 mg, at 15min, 1d, 3d, 7d, 14d, 28d, 42d
after injury, were homogenized in 1 ml TRIzol (Invitrogen), and mRNA was
extracted and purified by RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. The qualities of RNA were determined by Agilent
2100. 1-3 μg qualified RNA were subjected to Illumina V2 RNAseq library
construction, followed by Hiseq2000 SE50bp Sequencing.