Dataset for Pulmonary Vascular Moldels - 3D Print Images

The dataset is organized into two folders. The first folder contains images of 3D-printed hydrogel structures fabricated using a custom-built 3D micro-stereolithography printing device. The images are separated in six subfolders, identified by the subfolder names and described in detail in the subfolder description. One subfolder contains the information of templates used for parametric exploration or derived from in vivo images of fibrilar matrix of pulmonary arteries in normortensive and hypertensive animals. Three subfolders contain images that demonstrate the approach to optimize 3D hydrogel printing features including resolution, fidelity, and swelling ratio using printing parameters such as ink composition, UV intensity and exposure time, which are identified by the filenames. The final two subfolders show the printed microgels for cell culture and the creation of vascular-mimic structures for studying the physiological differences between diseased and normal blood vessels. The second folder contains images that demonstrate pulmonary vascular smooth muscle cells cultured in 3D-printed hydrogels with the templates taken from hypertensive (HX) or normentensive (NX) artery samples. The cells were stained using immunofluorescent techniques with alpha-SMA or paxillin focal adhesion molecule as well as stained with (Ca2+)-sensitive dye to visualize calcium cycling in live cells for characterizing their contracile functions in response to vasoactive substances.本数据集分为两个文件夹。 第一个文件夹内包含通过定制3D微型立体光刻打印设备（3D micro-stereolithography printing device）制备的3D打印水凝胶结构图像。该数据集的图像被划分为六个子文件夹，以子文件夹名称标识，且各子文件夹的说明文档中对其包含内容有详细描述。其中一个子文件夹存储用于参数探索的模板信息，或是从正常血压与高血压动物的肺动脉纤维状基质的体内图像提取得到的模板相关信息。三个子文件夹内的图像展示了如何通过墨水成分、紫外光强度、曝光时间等打印参数，优化3D水凝胶的打印性能指标，包括分辨率、保真度与溶胀比，相关参数可通过文件名识别。最后两个子文件夹则展示了用于细胞培养的打印微凝胶图像，以及用于研究病变与正常血管生理差异的血管模拟结构的制备图像。 第二个文件夹内包含在以高血压（HX）或正常血压（NX）动脉样本制备的模板所制成的3D打印水凝胶中培养的肺动脉血管平滑肌细胞图像。上述细胞采用免疫荧光技术（immunofluorescent techniques）进行染色：以α-平滑肌肌动蛋白（alpha-SMA）或桩蛋白（paxillin）这类黏着斑分子作为标记物，同时使用钙敏感性染料（(Ca2+)-sensitive dye）对活细胞进行染色，以可视化活细胞内的钙循环过程，从而表征其对血管活性物质响应的收缩功能。