Table1_A census of actin-associated proteins in humans.XLSX

Actin filaments help in maintaining the cell structure and coordinating cellular movements and cargo transport within the cell. Actin participates in the interaction with several proteins and also with itself to form the helical filamentous actin (F-actin). Actin-binding proteins (ABPs) and actin-associated proteins (AAPs) coordinate the actin filament assembly and processing, regulate the flux between globular G-actin and F-actin in the cell, and help maintain the cellular structure and integrity. We have used protein–protein interaction data available through multiple sources (STRING, BioGRID, mentha, and a few others), functional annotation, and classical actin-binding domains to identify actin-binding and actin-associated proteins in the human proteome. Here, we report 2482 AAPs and present an analysis of their structural and sequential domains, functions, evolutionary conservation, cellular localization, abundance, and tissue-specific expression patterns. This analysis provides a base for the characterization of proteins involved in actin dynamics and turnover in the cell.

肌动蛋白丝在维持细胞结构以及协调细胞运动和细胞内物质运输中发挥着至关重要的作用。肌动蛋白不仅参与与其多种蛋白质的相互作用，而且与自身结合形成螺旋状纤维状肌动蛋白（F-actin）。肌动蛋白结合蛋白（ABPs）和肌动蛋白相关蛋白（AAPs）共同调控肌动蛋白丝的组装与加工，调节细胞内球形G-actin与F-actin之间的动态平衡，并有助于维持细胞的结构与完整性。本研究利用来自多个来源（STRING、BioGRID、mentha等）的蛋白质-蛋白质相互作用数据、功能注释以及经典肌动蛋白结合域，鉴定了人源蛋白质组中的肌动蛋白结合蛋白和肌动蛋白相关蛋白。在此，我们报道了2482种AAPs，并对其结构域、序列特征、功能、进化保守性、细胞定位、丰度和组织特异性表达模式进行了分析。此项分析为细胞内肌动蛋白动态变化和周转所涉及蛋白的特征描述奠定了基础。