Single cell analysis of Monolayer and Organoid Primary Prostate Human Epithelial Cells Derived from the same patient. Single cell analysis of Monolayer and Organoid Primary Prostate Human Epithelial Cells Derived from the same patient

Single human primary prostate epithelial cells grown as a monolayer (2D) or as organoids (3D) were separated, collected and prepped for RNA-seq using the 10x Genomics Chromium Genome Reagent kit v2 Chemistry. 3,500-5,000 cells were collected from each sample and sequenced at a depth of ~45,000 genes per cells on a 2x150nt lane in a HiSeq 4000. CellRanger alignment was performed to produce the RAW data files. Overall design: Two samples: monolayer cells and organoid cells