RNA-sequencing analysis gene expression changes of H9C2 treated with CIRBP in response to oxygen-glucose deprivation/reperfusion

Cardiac arrest (CA) represents a significant public health concern, placing a substantial burden on the healthcare system. Post-restoration of autonomous circulation, a majority of patients encounter myocardial dysfunction, a primary cause of death in individuals with cardiac arrest. Hence, the identification of novel therapeutic approaches to enhance myocardial resilience in high-risk cardiac arrest patients holds immense clinical importance. Cold-inducible RNA-binding protein (CIRBP) serves as a vital player in cellular protection and stress resistance, induced by cold conditions. Studies have indicated that the elevation of CIRBP through its agonists can impede the progression of heart failure. However, its role in cardiac toxicity prompted by cardiac arrest remains ambiguous. This study delves into investigating the protective mechanism of CIRBP on cardiac function post-cardiac arrest and sheds light on potential target genes regulated by CIRBP via transcriptome sequencing. Our research outcomes highlight the robust cardioprotective impact of CIRBP on cardiac dysfunction triggered by cardiac arrest and resuscitation. Overall design: H9C2 were used as the research subjects. Comparative gene expression profiling analysis of RNA-seq data was performed on H9C2s treated with CIRBP after oxygen-glucose deprivation/reperfusion (OGD/R) . Each group consisted of three duplicate samples. The divided experimental groups was as follows: OGD/R + NC rep1 ,OGD/R+ NC rep2,OGD/R + NC rep3, OGD/R + CIRBP rep1, OGD/R + CIRBP rep2,OGD/R + CIRBP rep3, OGD/R + si-NC rep1,OGD/R + si-NC rep2, OGD/R + si-NC rep3,OGD/R + si- CIRBP rep1,OGD/R + si- CIRBP rep2,OGD/R + si- CIRBP rep3