Treatment of Pneumocystis murina with CuSO4

The discovery of specific copper-binding proteins and the differential gene expression in response to excessive copper exposure are significant contributions to the growing understanding of the adaptation mechanisms of extracellular pathogens in copper-rich environments, such as mammalian lungs. This project aims to elucidate the molecular mechanisms of copper tolerance and homeostasis in Pneumocystis murina, a crucial adaptation that allows the fungus to withstand host-induced copper cytotoxicity. This RNA-seq data enables identifying the gene cluster within P. murina that responds to excessive labile copper, thereby contributing to our understanding of its survival tactics in the hostile environment of the mammalian lung. By characterizing these adaptation mechanisms, we intend to uncover the fungus's adaptation strategies, potentially identifying new virulence factors and drug targets.