Boreal toad captivity and reintroductions

As captive assurance populations and reintroduction programs increase, a better understanding of how microbial symbionts respond during animal translocations is critical. We examined changes in microbes associated with boreal toads (Anaxyrus boreas boreas), a threatened amphibian, after reintroduction to the wild following captive rearing. Previous studies demonstrate that developmental life stage is an important factor in their microbiomes. We collected 16S marker-gene sequencing datasets to investigate: i) comparisons of the skin, mouth, and gut bacteria of boreal toads across four developmental life stages in captivity and the wild, ii) tadpole skin bacteria before and after reintroduction to the wild, and iii) adult skin bacteria during reintroduction to the wild. We demonstrated that differences occur across the skin, gut, and mouth microbial communities in captive versus wild boreal toads, and that the degree of difference depends on developmental stage.