Spatial transcriptomics reveals the pharmacological effects on tumors and TME structural heterogeneity under MASK vaccine treatment

To explore the pharmacological effects on tumors and TME structural heterogeneity under Magnetic Sculpture-liKe (MASK) Cells vaccine treatment, we performed spatial transcriptomics (ST) with B16F10 xenograft receiving MASK vaccine and magnet treatment. C57BL/6 mice subcutaneously inoculated with B16-F10-Luciferase cells were randomly divided into two groups: PBS and MASK. Sample MASK1 was collected on the 7 days after MASK vaccine treatment, Sample MASK2 was collected on the 13 days after MASK vaccine treatment. Magnet processing group according to the previous method. After the treatment periods, tumor samples were collected, and spatial transcriptomics was performed.