Relative expression of rev RNAs in subtype C viruses according to peak areas in GeneMapper analyses.

Results correspond to peaks shown in Fig. 2, and are shown as % of individual peak areas relative to the sum of peak areas of all rev RNA-derived products. Percentages at the column on the right correspond to the cloned and sequenced rev RNA-derived amplicons (Table 1).1A small 331 nt peak, coincident with that of 1.4g.7 rev RNA, was seen in X1702-3 and X1936 (Fig. 2). However, nested PCR using an antisense primer specific for rev, tat and vpr RNAs failed to detect 1.4g.7 rev RNA in these isolates.2Nested PCR with primers recognizing exons 2 and 3 allowed to confirm that these products, only 1 nt longer in X1702-3 and X1936 than in X2363-2, correspond to 1.3.4f.7 in the first two viruses and to 1.2.4g.7 in the third one.3The 367 nt peak seen in X2363-2 may correspond to both 1.2.4c.7 and 1.3.4b.7 rev RNAs. Nested PCR using primers recognizing exons 2 and 3 allowed to determine that this peak corresponds to 1.2.4c.7.