Figure 5

BMK-DKO cells expressing CBcl-W, transfected with VBH3 proteins treated with BH3 mimetics. Data displayed in Figure 5a-d was generated from combining results of 4 screens: 1) mim1_BclW_screen {Rep1_20190212, Rep2_20190213, Rep3_20190214, Rep4_20190215} 2) mim2_BclW_screen {Rep1_20190403, Rep2_20190404, Rep3_20190405, Rep4_20190406} 3) XL2W_screen {Rep1_20191108, Rep2_20191109, Rep3_20191110, Rep4_20191111} Figure 5f-g was generated from BH3 swap screen {Rep1_20200821, Rep2_20200827, Rep3_20200902, Rep4_20200904} -For each screen, see \"Master Platemap\" for screen design as well as data generated in qF3 analysis step 8_CombineBinnedReps: Here are, \"combined_WellID_binnedReps_.csv\" data files, that were fit to determine live cell Kds. Exported result from fitting these data is also included in, \"CombinedBinnedReps_Results.xlsx\". -Figure 5a-c shows the average of controls (VBH3 proteins and corresponding collisional control: VBH3-4E) included in each of these screens. (See \"Summarized_CBclW_ Results.xlsx\").