Nuclear Protein Aggregates Disrupt RNA Processing and Alter Biomechanics in a Muscle Cell Model of OPMD

RNAsequencing in three subcellular fractions: cytosolic, nuclear and insoluble from vehicle-treated cells and Dox-treated cells that induces the expression of the expanded (A16) PABPN1. The goal of this study was to demonstrate PABPN1 function in A16 cells, and to identify RNA accummulation in each fraction. This allows the identification of molecular signatures. Samples are from cytosolic, nuclear, and insoluble fractions isolated from vehicle and Dox-induced Ala16-PABPN1 in muscle cells. Biological replicates are N=3.