Rodent Research-1 (RR1) NASA Validation Flight: Mouse soleus muscle transcriptomic and epigenomic data

NASA's Rodent Research (RR) project is playing a critical role in advancing biomedical research on the physiological effects of space environments. Due to the limited resources for conducting biological experiments aboard the International Space Station (ISS), it is imperative to use crew time efficiently while maximizing high-quality science return. NASA's GeneLab project has as its primary objectives to 1) further increase the value of these experiments using a multi-omics, systems biology-based approach, and 2) disseminate these data without restrictions to the scientific community. The current investigation assessed viability of RNA, DNA, and protein extracted from archived RR-1 tissue samples for epigenomic, transcriptomic, and proteomic assays. During the first RR spaceflight experiment, a variety of tissue types were harvested from subjects, snap-frozen or RNAlater-preserved, and then stored at least a year at -80C after return to Earth. They were then prioritized for this investigation based on likelihood of significant scientific value for spaceflight research. All tissues were made available to GeneLab through the bio-specimen sharing program managed by the Ames Life Science Data Archive and included mouse adrenal glands, quadriceps, gastrocnemius, tibialis anterior, extensor digitorum longus, soleus, eye, and kidney. We report here protocols for and results of these tissue extractions, and thus, the feasibility and value of these kinds of omics analyses. In addition to providing additional opportunities for investigation of spaceflight effects on the mouse transcriptome and proteome in new kinds of tissues, our results may also be of value to program managers for the prioritization of ISS crew time for rodent research activities.

NASA的啮齿动物研究（Rodent Research, RR）项目在推进太空环境生理效应的生物医学研究中发挥着关键作用。鉴于国际空间站（International Space Station, ISS）上开展生物实验的资源有限，必须高效利用机组人员时间，同时最大化高质量科学产出。NASA的GeneLab项目核心目标有二：一是通过多组学（multi-omics）、基于系统生物学（systems biology）的方法进一步提升此类实验的研究价值；二是无限制地向全球科学界开放共享相关数据。本研究针对存档的RR-1组织样本中提取的RNA、DNA与蛋白质的活性进行评估，以用于表观基因组学（epigenomic）、转录组学（transcriptomic）及蛋白质组学（proteomic）分析。在首次RR太空飞行实验期间，研究人员从实验小鼠体内采集了多种组织样本，经快速冷冻或RNAlater保存液处理后，返回地球并在-80℃条件下储存至少一年。随后基于这些样本在太空研究领域的重大科学价值潜力，对其进行了优先级排序。所有组织样本均通过由艾姆斯生命科学数据档案馆（Ames Life Science Data Archive）管理的生物标本共享计划提供给GeneLab，涵盖小鼠肾上腺、股四头肌、腓肠肌、胫骨前肌、趾长伸肌、比目鱼肌、眼部组织及肾脏。本文报告了本次组织提取实验的方案与结果，由此证实此类组学分析的可行性与应用价值。除了为探索太空环境对小鼠转录组与蛋白质组的影响提供全新的组织类型研究契机外，本研究结果还可为项目管理者优化分配国际空间站机组人员用于啮齿动物研究活动的时间提供参考依据。

搜集汇总

背景与挑战

背景概述

该数据集来自NASA的Rodent Research-1验证飞行任务，专注于小鼠比目鱼肌的转录组学和表观基因组学数据，旨在评估从太空飞行后存档组织中提取RNA、DNA和蛋白质的可行性。它通过多组学分析研究太空环境对小鼠生理的影响，并为国际空间站的生物实验优先级提供科学依据，数据由GeneLab项目无限制共享给科研社区。