Analysis of TC-mAb mice (Exp2: Transitonal B, Mature B))

The experiment had been performed to analyze B cell development of human antibody-procducing mice (TC-mAb mice). The flow cytometry experiment was designed to detect the subsets of Follicular B (CD19+CD93-CD21loCD23hi), marginal zone B (CD19+CD93-CD21hiCD23lo) cells, transitional (T1 (CD19+CD93+IgMhiCD23-), T2 (CD19+CD93+IgMhiCD23+), and T3 (CD19+CD93+IgMloCD23+) in Spleen and Lymph node. In addditon, the expression pattern of IgM and IgD in Igk+ follicular B cells was also analyzed. Notes: This experiment corresponds to Figure 7b, c and Supplementary Figure 17-20 in the paper "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci". Publication link: https://doi.org/10.21203/rs.3.rs-103241/v1