Small-Molecule-Driven Direct Reprogramming of Fibroblasts into functional Sertoli-like cells as a model for male reproductive toxicology

We report the generation of functional Sertoli-like cells from mice fibroblasts using only chemical cocktails, I-bet151 and Riluzole (IR). CiSCs possess similar gene expression profiles and biological functions to endogenous Sertoli cells and are highly sensitive to reproductive toxicants. A total of 6 samples（2 CiSCs, 2 MEFs, 2 MSCs） were tested using the BGISEQ platform for this project to prove that the CiSC resemble Sertoli cell in genome-wide gene expression profile.