In the ciliate Paramecium tetraurelia, an efficient RNAi response can be triggered by feeding cells with bacteria producing double-stranded RNA against an endogenous gene. Some of the genes involved a
Small RNA produced by Dicer (Dcr1) are used to map dsRNA in wild-type strain and a xrn1-delta mutant of S. cerevisiae, inactivated for the cytoplasmic 5'-3' RNA decay pathway. Overall design: Small RN
We employed PSTVd infecting tomato as a model system and applied comprehensive genome-wide analyses to dissect the host responses to pathogenic ncRNAs.