Spatiotemporal single cell transcriptomic analysis of human gut macrophages reveals multiple functional and niche-specific subsets

Colonic resections were obtained from patients operated for sigmoid colon cancer at Akershus University Hospital (Ahus, 1478 Lørenskog, Norway). The resected colon was immediately examined by an experienced pathologistand macroscopically normal colon, at least 10 cm from the tumor, were placed in vials with RPMI 1640 and put on ice for transport. The study was performed in accordance with the Declaration of Helsinki. Written informed consent was obtained from all participants, and the study was approved by the Regional Committee for Medical Research Ethics (REK, 2018/703, Health Region South-East, Norway). For scRNA-seq, 4 colon specimens from sigmoid or ascending colon were obtained (age 62-78, 3 males). Immunofluorescence stainings were done on samples from 8 patients (age 62-78, 4 males) from ascending, transverse, descending and sigmoid colon. None of the patients had autoimmune, infectious or inflammatory diseases, nor received neoadjuvant chemotherapy or radiation therapy before the operation. All patients were operated following the national guidelines.EGA study EGAS00001005377