Select hyperactivating NLRP3 ligands enhance the Th1/Th17-inducing potential of human type 2 conventional dendritic cells

Among human DCs, the cDC2 subset represents the major human DC subset that is transcriptionally and functionally licensed for inflammasome responses as well as inflammasome formation in the absence of pyroptosis. These findings may allow for the development of new approaches in the field of adjuvant and vaccine development and define the human cDC2 subpopulation as a prime target for the treatment of inflammasome-dependent inflammatory diseases. NANOSTRING cDC1s isolated from PBMCs of a healthy individual, 3h control-treated (3 replicates) cDC1s isolated from PBMCs of a healthy individual, 3h R848-treated (3 replicates) cDC2s isolated from PBMCs of a healthy individual, 3h control-treated (3 replicates) cDC2s isolated from PBMCs of a healthy individual, 3h R848-treated (3 replicates)