The result of applying the OKO method on eciML1515 model for overproducing 41 native metabolites of E. coli.

Columns A-D present the name, KEGG ID, CHEBI ID and formulae of each metabolite, respectively. The maximum production level of each metabolite at optimal growth rate is shown in column E. Column F presents the production level of the metabolite in the engineeered model, where the biomass level is preseneted in cloumn G. The numbers of Kcat that are required to increase and decrease are respectively given in columns H and I, and the sum of fold-changes for Kcats is given in column M. Choline is one metabolite mapped in eciML1515 for which the maximum production is zero in both the wild-type and the engineered model. So, this metabolite is excluded from further analysis. (XLSX)