Transcriptomic changes following CENP-E partial depletion in normal human fibroblasts

Being CENP-E function restricted to mitosis, its haploinsufficiency has been used to study the induced cell aneuploidy. However, the gene expression profile triggered by CENP-E reduction in normal cells has never been explored. To fill this gap, here we investigated whether a gene network associated with siRNAs-induced CENP-E 50% reduction and consequent aneuploidy exists CENP-E was partially depleted by RNA interference in human primary fibroblasts (IMR90) and high-throughput gene expression profiling by DNA microarrays was done at “early” and “late” timepoints to investigate what gene/networks are engaged by CENP-E reduction