Adaptation of Acidithiobacillus spp. co-culture to arsenopyrite

The colombian indigenous iron oxidizing strain Acidithiobacillus ferrianus (IBUN Ppt12) and the sulfur oxidizing strain Acidithiobacillus sp. (IBUN Pt1247 S3) were selected to build up a bacterial consortium in a ratio 1:1 and then growth them on refractory ore composed mainly by arsenopyrite with a pulp density up to 10 % at 30 °C. In the course of the adaptation to increasing pulp concentrations in medium, it was extracted the total RNA from the consortium, getting samples of three different conditions, upon the arsenopyrite concentration (Fe+S / Fe+S+FeAsS 2% / FeAsS 3.33%). The transcriptomic profile was determined with RNA-seq to identify differentaily expresed genes during the adaptation process.