3D analysis of virus shape and NC length at different steps of filamentous MARV budding.

3D measurements of filamentous virus dimensions and identification of filamentous virus end shapes were carried out in 3D tomographic reconstructions of MARV-infected HUH-7 cells at 1 day p.i.. NC length measurements were taken in 3D on NCs completely included in tomographic reconstructions of MARV-infected HUH-7 cells at 1 and 4 days p.i.. All NCs in the cytoplasm, in budding structures, in filamentous viruses and in spherical particles have the same average length. *excluding one virus outlier of double length (1518 nm). **excluding one nucleocapsid outlier of double length (1472 nm), and two particles where the NC ends could not be reliably defined.