Trichoderma atroviride small RNA1 targets the Arabidopsis PRIM2 gene to establish a mutualistic relationship

This study investigates the multifaceted defense mechanisms of plants in response to beneficial fungi Trichoderma atroviride. The researchers focus on a small RNA, Ta_sRNA1, produced by T. atroviride during its interaction with Arabidopsis thaliana. A. thaliana roots inoculation with T. atroviride was performed and then collected from the interaction zone at 24, 48, 72, and 96 h of coculture. Total RNA was extracted and sRNA-seq libraries were generated according to the sRNA cloning protocol provided by Solexa.