Gene expression profile at single cell level of gingiva and alveolar bone tissues of canines with peri-implantitis and healthy conditions

Peri-implantitis is a common complication characterized by chronic inflammation of the periodontal tissue. This disease is marked by irreversible and progressive degradation of the gingiva and alveolar bone, ultimately leading to tooth loss.Most current research has focused on the entire periodontal tissue, which restricts a comprehensive understanding of the heterogeneity between gingiva and bone tissues, and hinders the development of targeted host therapies for peri-implantitis. To uncover the pathogenic mechanisms of peri-implantitis, our study employed a tissue-specific approach to investigate the interactions between stromal and immune cells in gingiva and bone tissues separately, using single-cell sequencing techniques. This strategy aims to develop the insights of the pathogenesis of peri-implantitis, providing a scientific basis for the treatment of peri-implantitis. The bilateral mandibular second, third and fourth premolars of one Beagle dog were extracted simultaneously for the further implant. The left mandibular teeth were used for the conduction of peri-implantitis model, while the right teeth were used for the healthy control. Gingva and bones tissues were obtianed for single-cell sequencing after an 8-week observation.