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J. R. Paulson, U. K. Laemmli, Don W Fawcett (2011) CIL:11021, Homo sapiens. CIL. Dataset

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资源简介:
Transmission electron micrograph of isolated HeLa cell metaphase chromosome decondensed by removal of histones with dextran sulfate and heparin, centrifuged onto a carbon film and stained with uranyl acetate. The residual chromosome 'scaffold' is surrounded by extended free DNA loops 20 to 24 micrometers long. Area boxed is shown at higher magnification in the grouped CIL image 11023.

高分辨率透射电子显微镜图像,展示了通过去除组蛋白并使用右旋糖酐硫酸盐和肝素进行去凝集的孤立HeLa细胞有丝分裂中期染色体,经离心沉积于碳膜上,并用铀酰乙酸染料染色。剩余的染色体‘支架’周围环绕着20至24微米长的游离DNA环。图像中框定的区域在11023组CIL图像中以更高的放大倍数呈现。
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