Confocal and fluorescence microscopy files of growing Pseudomonas putida microcolonies

<p>Description of the project</p>
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<p>The project aims at describing the formation and development of small bacterial colonies. Our hypothesis is that, along with nutrient and environmental conditions, the physical interactions between bacterial cells help shaping the micro colonies.</p>
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<p>Description of the dataset</p>
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<p>The dataset is divided into three folders, according to different experimental measurements. The first one (termed Fig1) contains the microscopy file where small colonies on a surface start developing from 1 single cell into micro-colonies. The second one (termed Fig2) contains two microscopy files, one that measures the dynamic formation of a colony and the other one that captures the transversal cut features (height, etc) of various segments. The third one contains raw figures taken from microscopy experiments under different agar concentrations.</p>
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<p>1. Methodology</p>
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<p>The growing cells on the agarose pad were recorded at 30 minutes time intervals using a Leica TCS SP5 confocal microscope equipped with a temperature control chamber. This approach allowed us to analyze 3D formation of cellular growth. We also cultivated the cell on the agarose pad overnight, and the growth formation in 2D was explored using an Olympus BX61 instrument. Green fluorescent signals were obtained with filters carrying an excitation laser (488 nm) built in both microscopes.</p>