Analysis of transcriptomes of germinal center (GC) B cells derived from miR-155-sufficient and -deficient mice

To investigate the impact caused by miR-155 ablation, positively selected light zone (LZ) GC B cells were sorted by flow cytometry and used to analyse transcriptomic profiles. Overall design: Splenic B cells derived from miR-155-sufficient or -deficient mice expressing HEL-specific B cell receptor together with endogenous cMyc-GFP reporter cassette were adoptively transferred into congenic mice, followed by immunization with HEL3x mutant conjugated with sheep red blood cells (SRBCs). After five days, cMyc-GFP+ HEL+ LZ GC-B cells were sorted and used for the mRNA sequencing library.