Early transcriptional landscapes of Chlamydia trachomatis-infected epithelial cells at single cell resolution

Here we examined host cell gene expression in response to Chlamydia trachomatis infection by applying scRNA-Seq to in vitro C. trachomatis-infected HEp-2 epithelial cells and time-matched uninfected cells over the early chlamydial developmental cycle (3, 6 and 12 hours). We collected 264 single cells across both conditions all time points. The aims of the experiment were examining host cell responses to infection at single cell resolution, and identifying early host cell signatures of infection. Examination of chlamydial infection (Chlamydia trachomatis) on human epithelial cells (HEp2) from three early time points using both infected and uninfected samples applying single cell RNA-Sequencing