Gene redundancy for cobalamin and tetrahydrofolate metabolism in M. extorquens CM4.

aHomologs with >90% aa Id (with mentioned exceptions) found in the chromosome of all M. extorquens strains AM1, BJ001, DM4, and PA1 (common core genome), in one of the strains (shared accessory genome), or none of these strains (CM4 specific CDS). The accessory genome includes a btuB homolog (Mpop_3807, 65% aa Id) in strain BJ001. For strain AM1, a putative dihydrofolate reductase dfrB gene (META2_0242, 34 and 28% aa Id with DmrA and DfrA, respectively) is found in addition to the chromosomal gene; moreover, homologs to Mchl_1923 (META2_0462, 33% aa Id with the N-terminal domain), and CzcA2 (META2_1026, 85% aa Id with pCMU01 plasmid czcA2) are found.bMaGe annotation (https://www.genoscope.cns.fr/agc/microscope).cPrecursors are uroporphyrinogen III and 5,6-dimethylbenzimidazole.dn.d., not detected.eEncode for homologs of different length: CobA (267 aa)/CysG (485 aa); CobC2 (519 aa)/CobC (338 aa); PurU (287 aa)/PurN (219 aa).fIn M. extorquens strains, H4F is synthesized either de novo or salvaged from 5,10-methenyl-H4F, or 5- or 10-formyl-H4F [11], [72], [73].