The function of IQGAP3 in gastric cancer

The first is transcriptional profiling of siRNA-mediated knockdown of IQGAP3 in human gastric cancer cell line AGS, NUGC3, and Hs746T comparing with non-target siRNA transfected AGS, NUGC3, and Hs746T as a control. The aim of this experiment was to evaluate the functions of IQGAP3 in three different gastric cancer cell lines in vitro. The second is bulk RNA-sequence from subcutaneous tumors, which were injected NUGC3 shIQGAP3 knockdown cells and NUGC shContorl cells into immunodeficient mice (NSG mice). The aim is to evaluate the difference of transcriptional profiling in human cancer cells and mouse stromal cells of shIQGAP3 knockdown tumors compared to shContorol tumors. Overall design: Transcriptional profiling of IQGAP3 knockdown samples compared to control samples using gastric cancer cell lines like AGS, NUGC3, and Hs746T, and bulk tumors from NUGC3.