10X genomic single cell RNA-seq of WT and p47phox-KI lungs with acute lung injury

We performed single cell RNA sequencing of CD45-negative cells sorted from WT and p47phox-KI lungs with ALI to characterize the impacts of the loss of p47phox phosphorylation in blood cells on pulmonary endothelial and epithelial cells. We found many genes are pertinent to enhancement of pulmonary vasculature integrity, maintain alveolar roles, promote ATII cell proliferation and lower oxidative stress state in p47phox-KI lungs. Overall design: Compare gene expression in single cell level between WT and p47phox-KI lungs