Identification of a minimum immunogenic epitope within PpNLP.

Synthetic peptides derived from a PpNLP fragment spanning amino acid residues 84–131 were tested for their abilities to trigger ethylene formation in Arabidopsis leaves. EC50 values were determined based upon using at least six different peptide concentrations. Peptides c and j are as indicated in Figure 2. Peptides 1–14 represent truncated versions of PpNLP fragment 84–131. Peptides 15–38 are truncated versions of PpNLP fragment 84–131 in which single amino acid residues are replaced by alanine residues (in bold). Data represent means ± SD of three replicates. Assays were performed three times with similar results.Identification of a minimum immunogenic epitope within PpNLP.