CD21hi and CD21lo Memory B cell bulk RNAseq gene transcript FPKM values

CD21hiCD19int and CD21loCD19hi IgG+ memory B cells were sorted from frozen PBMCS from four healthy adults. Sorted cells were frozen in RNAzol (Molecular Research Center) and RNA extracted according to the manufacturer’s instructions. mRNA libraries were constructed as described (Sandler et al., 2014) and sequenced using the Illumina TruSeq PE paired-end cluster kit on a HiSeq 2000. Trimmomatic (version 0.22) (Bolger et al., 2014) was used to remove adapters and low-quality bases. The trimmed paired-end reads were mapped to the reference human genome (Hg19) using Tophat (version 2.0.8) with a reference annotation (Ensembl“Homo_sapiens.GRCh37.74.gtf”). Samples with low map rates were discarded. Transcript abundance was determined with Cufflinks (version 2.1.1). This table lists FPKM values for all genes in each of the two sorted populations from each of the four individuals.从4名健康成人的冷冻外周血单个核细胞（PBMCs）中分选得到CD21高表达、CD19中表达（CD21hiCD19int）与CD21低表达、CD19高表达（CD21loCD19hi）的IgG+记忆B细胞。分选后的细胞冻存于RNAzol试剂（Molecular Research Center公司）中，并严格依照制造商说明书提取总RNA。mRNA文库构建参照已发表方法（Sandler等，2014）完成，随后采用Illumina TruSeq PE双端簇试剂盒在HiSeq 2000测序平台上进行双端测序。使用Trimmomatic（版本0.22）（Bolger等，2014）去除测序接头序列与低质量碱基。将质控后的双端测序reads比对至人类参考基因组（Hg19），比对工具为Tophat（版本2.0.8），参考注释文件采用Ensembl发布的"Homo_sapiens.GRCh37.74.gtf"。比对率较低的样本予以舍弃。使用Cufflinks（版本2.1.1）计算转录本丰度。本表格列出了4名个体的两个分选细胞群中所有基因的每千碱基百万片段数（FPKM, Fragments Per Kilobase of transcript per Million mapped reads）值。