Chlorophyll a data collected during the SIPEX II voyage of the Aurora Australis, 2012

Chlorophyll data was used to measure growth rates of sea ice algae in CO2 incubations. Sea ice brine microalgae was collected from sackholes. Replicate samples were incubated in ambient air (~0.04% CO2), 0.1% CO2, 1.0% CO2 and 2.0% CO2 concentrations. AT the end of the incubations the 50 ml samples were filtered through a 25 mm GF/F filter using vacuum filtration. The filters were placed in 15 ml plastic falcon tubes containing 10 ml of methanol, covered in aluminium foil and kept in the dark at 4 degrees C for 12 hours. Chl a concentration was measured using a 10AU Turner fluorometer following the acidification method of Strickland and Parsons (1972).
Data in spread sheet shows the extracted chl + phaeophytin, phaeophytin and chlorophyll concentrations (micro grams l-1) for each of the three experiments.

Data were collected at SIPEX Ice Stations 1-8 and SIPEX CTD stations 2-5