Purity of Primary Mouse Ear/Pinnae-derived Fibroblasts
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Primary fibroblasts were extracted from the ears/pinnae of three young C57BL/6 mice of each sex (male and female) from the NIA. Cells were frozen at the end of passage 3 and then thawed for analysis by flow cytometry. As a control, cells from a peritoneal lavage were collected and analyzed. Cells were stained for CD45, CD90.2, and CD140a to assess purity with a MACSQuant Analyzer 10. Data was analyzed with Flowlogic v7.<br>
创建时间：
2021-01-29
