Pacific spiny dogfish intestine amino acid transport

Datasets are presented for Weinrauch, Blewett, Anderson (In Prep.). See the 'metadata' text file for descriptions of individual .csv files and descriptions of column headings within. Experiments were conducted on male Pacific spiny dogfish (Squalus suckleyi) with approval from the Bamfield Marine Sciences Centre Animal Care Committee (protocol RS-21-03-R1) and collection permits from Fisheries and Oceans Canada (XR 264 2023) in Bamfield, BC, Canada in summer 2023. This dataset supported a study that examined the mechanisms of nutrient acquisition in the spiral valve intestine. To characterize the mechanisms of transport kinetic transport assays were fist conducted. To identify putative carriers of amino acids, additional fluxes were conducted in reduced sodium conditions (to deduce whether transport is sodium dependent) or alongside 100X the concentration of other amino acids known to utilise shared transport pathways in mammals. The interplay between amino acids and oleic acid was next assessed with the application of oleic acid to either the mucosal or serosal solutions. Finally, transport rates following feeding were measured. Together, this study provides new data on the mechanisms of dietary nutrient uptake and transport dynamics within an evolutionarily- and ecologically- important elasmobranch.本数据集配套于Weinrauch、Blewett与Anderson的在编研究（In Prep.）。请参阅"metadata"（元数据）文本文件，以获取各.csv文件及其内部列标题的详细说明。本研究于2023年夏季在加拿大不列颠哥伦比亚省班菲尔德开展，实验对象为雄性太平洋刺角鲨（Squalus suckleyi），实验流程已获得班菲尔德海洋科学中心动物护理委员会批准（协议编号RS-21-03-R1），并持有加拿大渔业与海洋部颁发的采集许可（许可编号XR 264 2023）。本数据集支撑了一项针对螺旋瓣肠道营养获取机制的研究。为解析营养转运机制，研究人员首先开展了转运动力学实验。为鉴定潜在的氨基酸转运载体，研究人员额外开展了两组通量实验：一组在低钠条件下进行，以推断转运过程是否依赖钠离子；另一组则添加了100倍浓度的已知在哺乳动物中共享转运通路的其他氨基酸。随后，研究人员通过将油酸（oleic acid）添加至黏膜侧或浆膜侧溶液中，探究了氨基酸与油酸之间的相互作用。最后，研究人员测定了动物摄食后的营养转运速率。综上，本研究为演化与生态均具有重要意义的软骨鱼纲（elasmobranch）物种的膳食营养摄取与转运动态机制提供了全新数据。