Analyses of red light-regulated genes by RNA-seq

Light, as both energy source and informational signal, profoundly influences plant growth and development during the whole life span from seed germination to flowering. To dissect the role for red light signaling in regulate the seedling development, we analyzed the gene expression profile of red light- and dark-grown WT seedlings by high throughput sequencing. WT seedlings were grown on 1/2 MS plates plus 1% sucrose under dark and 60 μmol m-2 s-1 red light. mRNA from each samples was sequenced to analyze the genes regulated by red light signaling.