FAM83H-AS1 is a non-coding oncogenic driver and therapeutic target of lung adenocarcinoma

Our study suggested that FAM83H-AS1 was a potential oncogenic driver due to chromosome 8q24 amplification in lung adenocarcinoma. To investigate the molecular mechanism of FAM83H-AS1, we performed high-thoughput RNA sequencing (RNA-Seq) assays after the silence of FAM83H-AS1 in A549 cell lines. Overall design: In human lung adenocarcinoma A549 cells, we downregulated the expression of FAM83H-AS1 utilizing small interference RNA (siRNA) and used scrumble as negative control. Then we used RNA-Seq to reveal the change of mRNA profiles after the silence of FAM83H-AS1.