Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion

The data are supplementary to the article "Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion" published in the International Journal for Parasitology 2022, vol. 52, issue 6, pp 343-358, https://doi.org/10.1016/j.ijpara.2021.12.003. The tables include mainly peptide identifications and annotations based on LC-MS/MS analyses of proteins contained in two separately isolated types of penetration glands of infectious larvae of the avian parasite Trichobilharzia szidati (Trematoda: Schistosomatidae) causing cercarial dermatitis in humans. Results of optimization experiments are also presented. Schistosome invasive stages, cercariae, leave their intermediate snail hosts, penetrate the skin of definitive hosts, and transform to schistosomula migrating to final location. During invasion, cercariae employ histolytic and other bioactive products of specialized holocrine secretory cells – postacetabular and circumacetabular penetration glands, the content of which is unsufficiently characterized. We present here the largest dataset known so far concerning the representation and localization of cercarial penetration gland proteins. We optimized the methods of sample processing with cercarial bodies (heads) first. Alizarin-pre-stained, chemically non-fixed samples provided optimal results of MS analyses, and enabled distinguishing PA and CA glands for laser microdissection. Peptidases of 5 catalytic types accounted for ca. 8 % and 6 % of reliably identified proteins in postacetabular and circumacetabular glands, respectively. Invadolysin, nardilysin, cathepsins B2 and L3, and an elastase 2b ortholog were the major gland endopeptidases. Two cystatins and a serpin were highly abundant peptidase inhibitors in the glands. While postacetabular glands generally had a rich enzymatic equipment, circumacetabular glands were conspicuously abundant in venom allergen-like proteins (PF00188 protein family members).本数据集为发表于《国际寄生虫学杂志（International Journal for Parasitology）》2022年第52卷第6期第343-358页、DOI为10.1016/j.ijpara.2021.12.003的论文《以宿主侵染相关蛋白为重点的禽毕睾吸虫尾蚴激光显微切割穿透腺差异蛋白质组学分析》的补充数据。数据集表格主要包含针对引发人类尾蚴性皮炎的禽类寄生虫斯氏毕睾吸虫（Trichobilharzia szidati，吸虫纲：裂体科）的两种分离纯化的穿透腺蛋白进行液相色谱-串联质谱（LC-MS/MS）分析所得的肽段鉴定结果与注释信息，同时收录了优化实验的相关结果。裂体科吸虫的侵染阶段——尾蚴会离开其中间宿主螺类，穿透终宿主皮肤并转化为童虫，最终迁移至最终寄生部位。在侵染过程中，尾蚴会借助特化的全泌分泌细胞（肛后腺与围肛腺）分泌的组织溶解物与其他生物活性产物，但目前对这些腺体的组分研究尚不充分。本数据集是目前已知规模最大的关于尾蚴穿透腺蛋白表达与定位特征的数据集。研究团队首先以尾蚴体部（头部）为样本优化了样品处理流程：经茜素预染色且未经过化学固定的样品可获得最优的质谱分析结果，同时可实现肛后腺（PA腺）与围肛腺（CA腺）的激光显微切割分离。在肛后腺与围肛腺的可靠鉴定蛋白中，5种催化类型的肽酶分别占比约8%与6%。主要的腺体内内肽酶包括Invadolysin、Nardilysin、组织蛋白酶B2与L3，以及弹性蛋白酶2b同源物。腺体内还存在两种高丰度的半胱氨酸蛋白酶抑制剂（cystatins）与一种丝氨酸蛋白酶抑制剂（serpin）。与酶系丰富的肛后腺不同，围肛腺中显著富集毒液变应原样蛋白（PF00188蛋白家族成员）。