18S DNA-metabarcoding method for terrestrial nematode suspensions

Nematodes are ideal biological indicators to monitor soil biodiversity and ecosystem functioning. With the increasing interest for agroecological studies, nematode communities are receiving more attention as a tool for soil health assessment by a broad range of scientists, increasing the need for widely accessible and quick methods for nematode identification. We investigated the potential use of DNA-metabarcoding based on amplicon sequencing for soil nematode community characterization. More specifically, we tested different DNA extraction methods and two 18S primer pairs on artificial nematode communities.