Ghosh, Rajarshi, Woodcock, Chris (2010) CIL:8045, Mus musculus. CIL. Dataset

Fluorescence recovery after photobleaching (FRAP)kinetics of MeCP2 were recorded using a mouse fibroblast cell line stably expressing MeCP2-GFP. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, followed by 107 images at 2 sec intervals.

利用稳定表达MeCP2-GFP的小鼠成纤维细胞系，采用荧光恢复后光漂白（FRAP）动力学技术进行了记录。通过Zeiss LSM 510元共聚焦显微镜，在488 nm激发波长下，使用63倍平面消色差油镜对活细胞进行成像。图像序列包含2次预漂白拍摄，一次是在对近端着丝粒异染色质1微米直径区域进行漂白后立即进行，随后以2秒间隔连续拍摄107张图像。