The construction of powerful cell factories requires intensive and extensive remodelling of microbial genomes. Considering the rapidly increasing number of these synthetic biology endeavours, there is
We optimized protocols for efficient multi-site mutagenesis of plasmids, reducing the time required from months to just one to two weeks while increasing the mutagenesis success rate for four distinct
This archive contains the optimized plqH genes used in the study "Heterologous plastoquinone production using a newly identified O2-dependent cyanobacterial hydroxylase", submitted to The FE
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery -Analysis of on-target and off-target editing at EMX1 and DYRK1A loci.