Transcriptomic analysis of neonatal supporting cells after histone modification perturbations
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https://www.ncbi.nlm.nih.gov/sra/SRP260320
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资源简介：
At neonatal stage, sensory hair cell gene promoters and enhancers are primed by H3K4me3/me1 in neighboring supporting cells, but silenced by deacetylation and H3K27 tri-methylation. To test whether this primed-but-silenced epigenetic status leads to the repressed expression of hair cell genes in supporting cells, we FACS-purified Lfng-GFP+ supporting cells from cultured postnatal day 1 (P1) ochlear organs 48 hours after TSA (HDAC inhibitor) or GSK-343 (EZH2 inhibitor) for transcritomic analysis by RNAseq. We found that hair cell genes could be induced by either blocking of histone deacetylation or inhibition of H3K27 trimethylation, suggesting histone deacetylation and H3K27 trimethylation are required to repress hair cell gene expression in supporting cells at neonatal stage when a latent transdifferentiation potential still exists in supporting cells. Overall design: Transcriptomes of supporting cells purified from DMSO, TSA or GSK-343 treated organs were analyzed by RNAseq
创建时间：
2021-10-09
