Biochemical analysis of H2O2-induced mutation spectra revealed that multiple damages were involved in the mutational process

This dataset is associated with the paper entitled "Biochemical analysis of H2O2-induced mutation spectra revealed that multiple damages were involved in the mutational process", by Sugiyama and Sanyal. "Fastq.zip" contains all fastaq raw data files that have been used in the paper. Fastaq file name contains NGS run name and barcode # (e.g. 22A_BC021.fastq). These identifications are used in Table S1 of the paper, which summarizes experimental conditions. "NGS Protocol 4.4.docx" is our laboratory protocol for basic analysis of our NGS data. This protocol is designed for new students to learn how to obtain mutation frequency from fastaq data. The protocol uses Excel template files with macro, which are also included in this dataset. The protocol and macros are designed for the specific data analysis, not for general use. Macro works only for specific template sequences used in the paper.