IL-13-mediates attenuation of LPS-induced BPIFA1 expression.

RPMI-2650 cells, either treated or untreated with IL-13 (10 ng/ml) for 48 h, were incubated with or without LPS (10 μg/ml) for further 2 h. Cell lysates were prepared to analyze the protein expression levels by using western blot. β-actin was used as the loading control. The western blots were carried out independently in triplicate and results were representative of one of three independent experiments. The expression level of each protein was quantified by signal intensity and was indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S4 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P