Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution

Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Descriptor: (3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside, 16S Ribosomal RNA, 23S Ribosomal RNA, ... Authors: Almutairi, M.M, Svetlov, M.S, Hansen, D.A, Khabibullina, N.F, Klepacki, D, Kang, H.Y, Sherman, D.H, Vazquez-Laslop, N, Polikanov, Y.S, Mankin, A.S. Deposit date: 2017-07-20 Release date: 2018-02-14 Last modified: 2025-03-19 Method: X-RAY DIFFRACTION (2.6 Å) Cite: Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins. Nucleic Acids Res., 45, 2017

分辨率为2.6埃的与匹克霉素（pikromycin）形成复合物、并结合信使RNA（mRNA）以及A、P、E位点转运RNA（tRNAs）的嗜热栖热菌（Thermus thermophilus）70S核糖体晶体结构
描述符：(3R,5R,6S,7S,9R,11E,13S,14R)-14-乙基-13-羟基-3,5,7,9,13-五甲基-2,4,10-三氧代-1-氧杂环十四烷-11-烯-6-基 3,4,6-三脱氧-3-(二甲氨基)-β-D-木己吡喃糖苷、16S核糖体RNA（16S Ribosomal RNA）、23S核糖体RNA（23S Ribosomal RNA）等
作者：Almutairi, M.M、Svetlov, M.S、Hansen, D.A、Khabibullina, N.F、Klepacki, D、Kang, H.Y、Sherman, D.H、Vazquez-Laslop, N、Polikanov, Y.S、Mankin, A.S
提交日期：2017-07-20
发布日期：2018-02-14
最后修改日期：2025-03-19
实验方法：X射线衍射（X-RAY DIFFRACTION，2.6 Å）
引用文献：天然联合产物酮内酯类化合物甲基霉素（methymycin）与匹克霉素通过抑制有限数量蛋白质的合成阻断细菌生长。《核酸研究》（Nucleic Acids Res.）, 45, 2017