Doxorubicin induces the senescence-associated secretion phenotype in RAW264.7 cells

Doxorubicin is a widely used anti-cancer drug, and its toxicological effects on healthy tissues, especially the heart, remains challenging in therapy. To explore the potential targets that ameliorate doxorubicin-induced cardiotoxicity (DIC), we aimed to investigate the role that macrophages play in the DIC development. Senescence of RAW264.7 cells was induced through doxorubicin administration in the experiment, and the transcription of secretory components was analyzed in this study. Overall design: RAW264.7 cells were treated by medium and medium plus 100nM doxorubicin for 24h (n=5). [The concentration in the samples should be 100nM]